diff --git a/unittests/cfdp/handler/testSourceHandler.cpp b/unittests/cfdp/handler/testSourceHandler.cpp
index e7f62def..c268130b 100644
--- a/unittests/cfdp/handler/testSourceHandler.cpp
+++ b/unittests/cfdp/handler/testSourceHandler.cpp
@@ -2,6 +2,7 @@
 
 #include <catch2/catch_test_macros.hpp>
 #include <filesystem>
+#include <random>
 
 #include "fsfw/cfdp.h"
 #include "fsfw/cfdp/handler/PutRequest.h"
@@ -26,6 +27,8 @@ TEST_CASE("CFDP Source Handler", "[cfdp]") {
   using namespace cfdp;
   using namespace returnvalue;
   using namespace std::filesystem;
+  const size_t MAX_FILE_SEGMENT_SIZE = 255;
+
   MessageQueueId_t destQueueId = 2;
   AcceptsTmMock tmReceiver(destQueueId);
   MessageQueueMock mqMock(destQueueId);
@@ -48,6 +51,7 @@ TEST_CASE("CFDP Source Handler", "[cfdp]") {
   auto sourceHandler = SourceHandler(dp, fp);
 
   RemoteEntityCfg cfg;
+  cfg.maxFileSegmentLen = MAX_FILE_SEGMENT_SIZE;
   EntityId id(cfdp::WidthInBytes::TWO_BYTES, 5);
   cfg.remoteId = id;
   std::string srcFileName = "/tmp/cfdp-test.txt";
@@ -138,6 +142,8 @@ TEST_CASE("CFDP Source Handler", "[cfdp]") {
 
     // Verify metadata PDU was sent.
     genericMetadataCheck(fsmResult, expectedFileSize);
+
+    // Verify that a small file data PDU was sent.
     fsmResult = sourceHandler.stateMachine();
     TmTcMessage tmtcMessage;
     const uint8_t* pduPtr;
@@ -167,5 +173,61 @@ TEST_CASE("CFDP Source Handler", "[cfdp]") {
     CHECK(sourceHandler.getState() == CfdpState::IDLE);
   }
 
-  SECTION("Transfer two segment file") {}
+  SECTION("Transfer two segment file") {
+    // Create 400 bytes of random data. This should result in two file segments, with one
+    // having the maximum size.
+    std::random_device dev;
+    std::mt19937 rng(dev());
+    std::uniform_int_distribution<std::mt19937::result_type> distU8(0, 255);
+    std::array<uint8_t, 400> largerFileData{};
+    for (auto& val : largerFileData) {
+      val = distU8(rng);
+    }
+    size_t expectedFileSize = largerFileData.size();
+    fsMock.createFile(srcFileNameFs);
+    FileOpParams params(srcFileName.c_str(), expectedFileSize);
+    fsMock.writeToFile(params, reinterpret_cast<const uint8_t*>(largerFileData.data()));
+    CHECK(sourceHandler.transactionStart(putRequest, cfg) == OK);
+    SourceHandler::FsmResult& fsmResult = sourceHandler.stateMachine();
+    // Verify metadata PDU was sent.
+    genericMetadataCheck(fsmResult, expectedFileSize);
+
+    // Check first file data PDU. It should have the maximum file segment size.
+    fsmResult = sourceHandler.stateMachine();
+    TmTcMessage tmtcMessage;
+    const uint8_t* pduPtr;
+    FileDataInfo fdInfo;
+    auto accessor = onePduSentCheck(fsmResult, tmtcMessage, &pduPtr);
+    {
+      FileDataReader fdReader(pduPtr, accessor.second.size(), fdInfo);
+      // 10 byte PDU header, 4 byte offset, 255 byte file data
+      CHECK(accessor.second.size() == 269);
+      CHECK(fdReader.parseData() == OK);
+      CHECK(fdInfo.getOffset().value() == 0);
+      size_t fileSize = 0;
+      const uint8_t* fileData = fdInfo.getFileData(&fileSize);
+      // Maximum file segment size.
+      REQUIRE(fileSize == MAX_FILE_SEGMENT_SIZE);
+      for (unsigned i = 0; i < fileSize; i++) {
+        CHECK(fileData[i] == largerFileData[i]);
+      }
+    }
+    mqMock.clearMessages();
+
+    // Check second file data PDU.
+    fsmResult = sourceHandler.stateMachine();
+    accessor = onePduSentCheck(fsmResult, tmtcMessage, &pduPtr);
+    FileDataReader fdReader(pduPtr, accessor.second.size(), fdInfo);
+    // 10 byte PDU header, 4 byte offset, remaining file data (400 - 255 == 145).
+    CHECK(accessor.second.size() == 10 + 4 + largerFileData.size() - MAX_FILE_SEGMENT_SIZE);
+    CHECK(fdReader.parseData() == OK);
+    CHECK(fdInfo.getOffset().value() == MAX_FILE_SEGMENT_SIZE);
+    size_t fileDataSize = 0;
+    const uint8_t* fileData = fdInfo.getFileData(&fileDataSize);
+    // Maximum file segment size.
+    REQUIRE(fileDataSize == largerFileData.size() - MAX_FILE_SEGMENT_SIZE);
+    for (unsigned i = 0; i < fileDataSize; i++) {
+      CHECK(fileData[i] == largerFileData[MAX_FILE_SEGMENT_SIZE + i]);
+    }
+  }
 }
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